Hi Histonetters,
I just came across a thread regarding issues with Cytokeratin AE1/AE3 from
DAKO. I am having the same issues with our
New protocol using HIER on the Link-48 in which sentinel lymph nodes from
breast cases are staining non-specifically in-between the cells.
We never had this
Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use
the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a
protocol on sentinel lymph nodes from breast cases that were negative on frozen
section to rule out micro metastases and what my
] Cytokeratin AE1/AE3
Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We
use the antibody from DAKO and do HIER on it before staining. We run
AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were
negative on frozen section to rule out micro metastases and what
It may be specific labeling of extra-follicular dendritic cells that contain
low molecular weight cytokeratin and, if your IHC assay is truly optimized, you
won't be able to get rid of it. I use the LMW CK mAb clone 5D3 that labels
CK8/18 and I see this reactivity all the time. I like it
