[Histonet] DIF on paraffin embedded tissue

The response to a problem like this is that your lab has not validated such a procedure for diagnostic use so the results are completely unreliable with your current protocols. Sure, you can "try it" but a negative result would mean nothing at all. Or you might get massive background that also

Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.  We URGENTLY

Re: [Histonet] DIF on paraffin embedded tissue

Buesa <rjbu...@yahoo.com> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > N

Re: [Histonet] DIF on paraffin embedded tissue

@lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 9:10 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet > <

Re: [Histonet] DIF on paraffin embedded tissue

Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet > wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is > USELESS.René > > >On Wednesday, November 25, 2015 10:55 AM,

Re: [Histonet] DIF on paraffin embedded tissue

@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] DIF on paraffin embedded tissue I'm not sure that is the case in the grand scheme of things, it will depend upon the target that you need to stain via immunofluorescence. Technically we perform IF staining

[Histonet] DIF on paraffin embedded tissue

We have a tissue sample that was processed and paraffin embedded. We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure. HELP madeathri...@pastnashville.com ___ Histonet

Re: [Histonet] DIF on paraffin embedded tissue

onet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] DIF on paraffin embedded tissue I, agreeing with Liz, would suggest looking out a IF protocol for FFPE tissues - I've recently concluded a validation of PLA (proximity ligation assay) in: cells in culture,

Re: [Histonet] DIF on paraffin embedded tissue

No way...no how Sorry! Sent from my iPad > On Nov 25, 2015, at 11:10 AM, Simmons, Christopher via Histonet > wrote: > > Useless sample > Sorry > > Sent from my iPhone > >> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet >>

Re: [Histonet] DIF on paraffin embedded tissue

5 11:02 AM To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge vi

Re: [Histonet] DIF on paraffin embedded tissue

Maryann, Whoa, bad day. I'm sorry for you and for the patient. As for whether the testing usually performed in frozen section by Direct ImmunoFluorescence can be successfully done on FFPE, Liz Chlipala is probably the closest to answering this. This was likely a kidney or skin biopsy. The

Re: [Histonet] DIF on paraffin embedded tissue

02 AM > To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] DIF on paraffin embedded tissue > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is > USELESS.René > > >On Wednesday, November 25, 2015 10:5

Re: [Histonet] DIF on paraffin embedded tissue

-Original Message- From: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 11:30 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] DIF on paraffin embedded tissue Maryann, Whoa, bad day. I'm sorry for you and for the patient