Re: [Histonet] Dirty H after break

2017-01-08 Thread Tony Henwood (SCHN) via Histonet
...@gmail.com] Sent: Saturday, 7 January 2017 4:01 PM To: Tony Henwood (SCHN) Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dirty H after break Yep, filtering the hematoxlyn fixed it. Thanks for the tip. Is it worthwhile to filter the eosin, alcohols, and xylene substitutes as well

Re: [Histonet] Dirty H after break

2017-01-06 Thread Allan Wang via Histonet
t [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, 4 January 2017 4:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dirty H after break > > Hello all, > > Does someone know what would cause dirty flakes of light purple that > showed across the wh

Re: [Histonet] Dirty H after break

2017-01-03 Thread Tony Henwood (SCHN) via Histonet
] Sent: Wednesday, 4 January 2017 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dirty H after break Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh

[Histonet] Dirty H after break

2017-01-03 Thread Allan Wang via Histonet
Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't