[Histonet] Embedding paraffin for brain tissue?

2014-01-02 Thread Fawaz Zouabi
Hi All Before looking at the wax issue, have you tried changing/looking at the processing issue first? We cut our human brain tissues (routine and large ) daily and we don't have any problem . We found that brain specs are better processed on a 5 days cycle, making them easier to handle ,cut a

Re: [Histonet] Embedding paraffin for brain tissue?

2014-01-02 Thread Pirici Daniel
Street 2, 200349 Craiova, Dolj Romania From: Maria Mejia To: Tim Wheelock Cc: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 2, 2014 8:04 AM Subject: Re: [Histonet] Embedding paraffin for brain tissue? Hello, I too have been experi

Re: [Histonet] Embedding paraffin for brain tissue?

2014-01-01 Thread Maria Mejia
Hello, I too have been experiencing the same difficulties cutting brain samples, I've tried 3 different types of paraffins - all the the same rolling of sections, some lines & tearing. It's not my microtome, because I've had it checked & it went through a recent general maintenance. I've tried

[Histonet] Embedding paraffin for brain tissue?

2013-12-31 Thread Tim Wheelock
Hi Everyone: In November, there was a discussion concerning different types of embedding paraffin. Do people find that a certain kind of wax is preferable for embedding brain tissue, or doesn't it matter? I also use Surgipath EM-400, but have decided to try another brand. I have been using th