Chuckle
Thank you, John Kiernan
Yes...I appreciate your filling in them gaps.
I do understand/know themhaving read, over the years many of the Giants of
Histology who gave me such inciteful/usable knowledge, including yourself.
I just didn't want to bloat my "pennyworth" otherwise it would be
lner jars. I wonder
if anyone else has done this?
John Kiernan
Anatomy & Cell Biology
UWO, London, Canada
= = =
From: Hobbs, Carl via Histonet
Sent: 05 July 2020 14:25
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fixed frozen non-paraffin
Prof. Kiernan, as usual, provides us all with such a depth/breadth of
particular information/advice.
His Histological and Histochemical methods BIBLE is still my favourite read.
Respect
Most researchers fix in depolymerised Paraformaldehyde because someone must've
originally thought:
" Hang on,
chulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html
= = =
From: Roy, Edward J via Histonet
Sent: 04 July 2020 20:08
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fixed frozen non-paraffin mouse brain
As a research lab, we sometimes would like
Hi Ed,
I have been using the 30% sucrose technique to cryoprotect animal tissue
for over 40 years without any problem. Did the tissue sink to the bottom of
the specimens jar? After sinking, I blot the excess sucrose from the tissue
on a paper towel before transfering to OCT. What is your procedure
You need to add sucrose to your PFA we use 4%PFA+4%Sucrose to fix and then
cryoprotect in 30% sucrose which all need to be prepared in Phosphate buffer
solution NO saline if you message me directly I would be happy to share
our SOP this was a very hard thing to learn not a lot in techni
As a research lab, we sometimes would like to use paraformaldehyde-fixed but
non-paraffin embedded tissues; paraffin embedding alters antigens and
necessitates antigen retrieval, but simple fixation does not. We have done the
traditional 30% sucrose before OCT and freezing, with cryostat section
