...@gmail.com]
Verzonden: woensdag 6 juni 2012 1:31
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: Re: [Histonet] Fluorescence-filters
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good start
Of Emily Sours
Sent: Tuesday, June 05, 2012 7:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fluorescence-filters
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good
Hi!
Filters for fluorescencemicroscopy tend to burn out after a certain
duration of usage. What duration?
We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.
What do you think? Is it time to change them.
I have often bad feedback about weak
, but not of the filters.
If you are getting weaker signals perhaps is another cause.
René J.
From: Gudrun Lang gu.l...@gmx.at
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, June 5, 2012 10:37 AM
Subject: [Histonet] Fluorescence-filters
Hi!
Filters
: [Histonet] Fluorescence-filters
Hi!
Filters for fluorescencemicroscopy tend to burn out after a certain
duration of usage. What duration?
We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.
What do you think? Is it time to change them.
I have
AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fluorescence-filters
Hi!
Filters for fluorescencemicroscopy tend to burn out after a certain duration
of usage. What duration?
We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are
about 150 per year
Hi Gudrun,
i read about 15 ALK cases a week. If you are seeing a lot of collagen
fibers around the tumor cells, I'd try increasing the digestion time of
your pepsin (especially if they were fixed for longer than usual). Before
altering the pretreatment though, you would want to make sure that
Wow, that was detailed and interesting!!
I didn't know microscopes could even use LED, does that require a different
setup, or just a different bulb?
Emily
You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh
