Would anyone be willing to share with me what you do with residual frozen
tissue from immunofluorescence testing or frozen tissue remaining from muscle
biopsy? CAP requirements only address what you do with residual frozen tissue
remaining from intraoperative consultations. We keep the frozen
I'm testing a faster Mart 1 for melanoma on Mohs specimens. Its been a while
since I've done any frozen tissue IHC stains and I can't remember the fixation
to keep it viable for testing the next day, or same day but later on. Will it
hold up if fixed in acetone and dried or do I need to
Hello histonet,
I am looking for a frozen tissue training session for my new employee. It
includes frozen tissue harvest, storing, sectioning, etc. Could any one
recommend a training session inside US?
Thanks,
Amy
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Histonet mailing list
:32 PM
To: histonet
Subject: [Histonet] frozen tissue training
Hello histonet,
I am looking for a frozen tissue training session for my new employee. It
includes frozen tissue harvest, storing, sectioning, etc. Could any one
recommend a training session inside US?
Thanks,
Amy
It's almost Friday Histonetters!
What kinds of containers are you storing your OCT embedded samples in? We're
currently using snap top vials for all of our frozen samples that have a
tendency to pop their lids when removed from our LN2 freezers! Wondering if
there are some type of screw top
I am curious as to the frozen tissue retention policies in other institutions,
especially in children's facilities. How long are samples considered
diagnostically viable and relevant to patient care? What is done with the
tissue when the time limit is reached?
Our facility is interested in
Hi everyone,
We're having trouble with frozen tissue detachment (mouse aortic root).
Our procedure is as follows:
1. Fresh heart into NBF 4% (Lilly's) 24h, fridge (+4)
2. OCT (tissue-tek) 2h, fridge (+4)
3. Quickly frozen in icecold isopentan
4. Freezer (-20) until sectioned at 10 µm in
Hi everyone,
Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster. I appreciate everyone's help. Now I am back for more info. I
deleted the depar. and cell conditioning steps and I got specific staining
with little background. There was just one problem.the
To: Sheila Fonner
Subject: Re: [Histonet] Frozen Tissue Protocol
You are seeing endogenous phosphatase, not endogenous peroxidase. Use 10%
acetic acid for 1-2 minutes.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513
From the research point of view, I've heard of people not fixing tissue
before they section it. Since I work with embryonic tissue (which is mostly
water!), we always fix our tissue. It definitely is better to not fix tissue
when you want to stain with antibodies because anything you do to the
We have a new doctor in our lab who swears that all frozen tissue must
be fixed in formalin with a subsequent sucrose treatment before freezing
in OCT because not fixing it will cause the structures to be distorted
and you can't get good antibody attachment. In my previous experience,
we have
jreichensper...@siumed.edu
Subject: [Histonet] Frozen tissue question
To: histonet@lists.utsouthwestern.edu
Date: Thursday, June 23, 2011, 10:12 AM
We have a new doctor in our lab who swears that all frozen tissue must be fixed
in formalin with a subsequent sucrose treatment before freezing in OCT
I run a Mohs lab that processes skin by frozen section. There is no need
to use any fixative before hand. But, if you are looking for melanoma or
melanocytes, freezing can cause artifact and make it difficult to read
slides. Limit the amount of nitrogen you use to freeze the specimen. Some
places
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