Re: [Histonet] Frozens and antigen retrieval

2012-07-05 Thread Kim Donadio
@lists.utsouthwestern.edu Sent: Wednesday, July 4, 2012 7:02 AM Subject: [Histonet] Frozens and antigen retrieval Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody company lists the retrieval

[Histonet] Frozens and antigen retrieval

2012-07-04 Thread Daniela Bodemer
Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. Hit me with your opinions on

Re: [Histonet] Frozens and antigen retrieval

2012-07-04 Thread Lee Peggy Wenk
: Daniela Bodemer Sent: Wednesday, July 04, 2012 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozens and antigen retrieval Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody