This happens to us after extended use. Add about 5 drops/100ml of glacial
acetic acid to the light green working solution.
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I think that your leight green solutions is too diluted. Prepare a new solution
and try again.
René J.
--- On Fri, 10/7/11, Michele Carr wrote:
From: Michele Carr
Subject: Re: [Histonet] Green counterstain problems
To: rg...@bmnet.com, histonet@lists.utsouthwestern.edu
Date: Friday, October
I'm having the same problem, would be interested in what others say.
T-Mobile, America's First Nationwide 4G Network
rg...@bmnet.com wrote:
Good Morning Histonetters!
I've been having a counterstain problem with my GMS and PAS-F. Both stains
work as they should until the green counterstain i
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rg...@bmnet.com
Sent: Tuesday, October 04, 2011 10:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Green counterstain problems
Good Morning Histonetters!
I've been h
Good Morning Histonetters!
I've been having a counterstain problem with my GMS and PAS-F. Both stains
work as they should until the green counterstain is applied. I cannot get
my tissue (control or patient) to uptake the counterstain. I have varied
time, temperature, even companies. I have go