[Histonet] Her2 dual ISH

Hello I was reading the past posts of inconsistent her2 dual ISH results on the VMS Ultra. I am wondering if anyone has unlocked the secret of getting consistent results? Thank you advance! Ginny ___ Histonet mailing list

[Histonet] Her2-ish

Does anyone run her2 ish on the Leica Bonds. If so who do you purchase your antibody from? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Her2 Dual ISH

What CPT code is everyone that does Her2 Dual ISH use? There are a lot of codes for ISH to choose from. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557

Re: [Histonet] Her2 Reflex

Re: Her2 by IHC We reflex 1+ to FISH, too Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ___ Histonet mailing list

Re: [Histonet] Her2 IHC

My lab does not have a written policy on this yet but are discussing it. If the patient has a high grade tumor and HER2 IHC is 1+ (or even 0+), some of our pathologists will reflex to FISH. The oncologists will request it sometimes too, more often when ER and PR are both negative. We have found

[Histonet] Her2 IHC

Are any labs reflexing 1+ for FISH? Thanks :) Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.al...@providence.org This message

RE: [Histonet] Her2:

: [Histonet] Her2: Can anyone give me suggestions of what they are using for Her2? What are your thoughts on FDA cleared kits vs ASR? Good products/cost efficientgeneral thoughts. Also, does anyone know or have any literature stating that an FDA cleared kit has to be used on a specific

[Histonet] Her2:

Can anyone give me suggestions of what they are using for Her2? What are your thoughts on FDA cleared kits vs ASR? Good products/cost efficientgeneral thoughts. Also, does anyone know or have any literature stating that an FDA cleared kit has to be used on a specific platform that it is

[Histonet] Her2 IHC validation

In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates.We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have

RE: [Histonet] Her2 IHC validation

Yes, check the ASCO/CAP guidelines there are correlation rates for positives and negatives. Joelle Weaver MAOM, HTL (ASCP) QIHC From: mw...@wakehealth.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 22 Jan 2015 20:21:53 + Subject: [Histonet] Her2 IHC validation

[Histonet] HER2 IHC controls

Are any of you making your own HER2 IHC control slides from patient tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are

[Histonet] HER2 IHC score averages

Would anyone happen to know the overall scoring averages for HER2 IHC for each score (0, 1+, 2+, 3+)? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity

[Histonet] HER2 scoring exercises for breast cases

Does anyone know of a good website for practicing HER2 scoring on breast tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are

RE: [Histonet] HER2 by IHC- Fixation

in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, June 04, 2014 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 by IHC

[Histonet] HER2 by IHC- Fixation

Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman

RE: [Histonet] HER2

...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 13, 2014 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using

RE: [Histonet] HER2

Thank you Josie this is very helpful. Joelle Weaver MAOM, HTL (ASCP) QIHC Subject: RE: [Histonet] HER2 Date: Wed, 14 May 2014 07:22:39 -0400 From: jcbrit...@cheshire-med.com To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu We use Integrated Oncology-Lab Corp, 521 West

[Histonet] HER2

Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using Refine detection. My clone is the Cb11, Oracle. Thanks in advance for any information you can provide. Joelle Weaver MAOM, HTL (ASCP) QIHC

[Histonet] HER2/NEU

I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from

[Histonet] Her2 Staining

Good morning Histonetters: Our CAP/ NY certified Cancer diagnostic reference lab is going to start to offer Her2 staining for breast and gastic tissues using the DAKO Kit on the bench.  The staining was validated by myself and our RD group and the SOP will be transferred to clinical.  I

RE: [Histonet] Her2 Dual ISH and breast processing

Thank you for the references, I have found what I needed in this article: Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma. Goldstein NS, et. al., Am J Clin Pathol 2003;120:86-92 Willem My mistake: the web site is:

RE: [Histonet] Her2 Dual ISH and breast processing

Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtol...@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental

Re: [Histonet] Her2 Dual ISH and breast processing

Subject: Re: [Histonet] Her2 Dual ISH and breast processing Please go to http://www.histosearch.co/rene.html There are 2 articles on the subject: one about the general fixation issue and another about the minimum amount of NBF required to obtain complete fixation. René J. From: Hoekert, W.E.J

Re: [Histonet] Her2 Dual ISH and breast processing

; vtol...@cox.net; histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 7:35 AM Subject: RE: [Histonet] Her2 Dual ISH and breast processing I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno

[Histonet] Her2 Dual ISH and breast processing

Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue

Re: [Histonet] Her2 Dual ISH and breast processing

reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). René J. From: vtol...@cox.net vtol...@cox.net To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual

[Histonet] Her2 FISH controls

Hello, I see that the ASCO/CAP Guidelines recommend using amplified, cutoff, and normal controls for Her2 FISH testing. We currently use Pathvysion and purchase normal and cutoff controls from Abbott. They do not offer a product of amplified controls. Are you all running this third control? Are

AW: [Histonet] Her2 FISH controls

, Melissa Gesendet: Dienstag, 12. April 2011 15:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Her2 FISH controls Hello, I see that the ASCO/CAP Guidelines recommend using amplified, cutoff, and normal controls for Her2 FISH testing. We currently use Pathvysion and purchase normal

[Histonet] Her2 Gastric cases

Hello, I am hoping someone can shed some light on whether or not Her2 4B5 (Ventana's antibody) is okay to run on gastric biopsies. We are getting some client requests for this and I wonder if I should re-direct them toward the Dako Hercep Test instead. Thank you. Margaret G. Coppin,

RE: [Histonet] Her2 Gastric cases

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Coppin, Margaret Sent: Friday, March 11, 2011 9:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Gastric cases Hello, I am hoping someone can shed some light on whether or not Her2 4B5

Re: [Histonet] Her2 Gastric cases

We do use the Ventana antibody for gastric cases. There is some difference in reading the slide for the pathologist. I'll send you an article on it in a seperate e-mail (so I can attach it). Mark On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret copp...@aruplab.comwrote: Hello, I am

RE: [Histonet] Her2 Gastric cases

@lists.utsouthwestern.edu Subject: Re: [Histonet] Her2 Gastric cases We do use the Ventana antibody for gastric cases. There is some difference in reading the slide for the pathologist. I'll send you an article on it in a seperate e-mail (so I can attach it). Mark On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret

[Histonet] Her2 requests on decal specimens

I am hoping to get some guidance on this. Occasionally we get requests to perform HER2 on specimens that have been decaled. The revised CAP question, ANP.22998 specifically states in the notes that decalcification with strong acids should not be used. How are other institutions handling

RE: [Histonet] Her2 Fixation Requirement

02/03/2011 04:54 PM To Weems, Joyce jwe...@sjha.org, Paula Lucas plu...@biopath.org, histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Her2 Fixation Requirement Does anyone have issues with inking the specimen after it has been placed in formalin? -Original Message- From

[Histonet] Her2 Fixation Requirement Responses/Thanks

Your information and suggestions helped me formulate a plan, so thank you to those who took the time and responded to my inquiry yesterday. Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Her2 Fixation Requirement

Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document

[Histonet] Her2 Fixation Requirement

Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind

Re: [Histonet] Her2 Fixation Requirement

Subject [Histonet] Her2 Fixation Requirement 02/02/2011 12:11 PM

RE: [Histonet] Her2 Fixation Requirement

: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping

RE: [Histonet] Her2 Fixation Requirement

What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.dis...@hcahealthcare.com

RE: [Histonet] Her2 Fixation Requirement

, 2011 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett

RE: RE: [Histonet] Her2 Fixation Requirement

, February 02, 2011 14:16 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett

RE: [Histonet] Her2 Fixation Requirement

Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 02, 2011 2:28 PM To: lori.dis...@hcahealthcare.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement Lori

[Histonet] Her2 question for tissue processors

Are there any validation requirements for tissue processing Her2 neu specimens? Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572

[Histonet] HER2 - Automated Slide Reade

Does anyone use an automated slide reader for HER2 slides? Possibly in the market and would really appreciate some feedback on what system and associated comments? Thanks! Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197

[Histonet] her2 validation

sorry!!! I didn't put a subject in the previous post. wondering about how many slides people are using for her2 validation. thanks anita dudley providence hospital mobile alabama

Re: [Histonet] her2 validation

We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was

RE: [Histonet] Her2 fixation question

...@northside.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, October 29, 2009 5:17 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Her2 fixation

[Histonet] HER2 on upper GI biopsies

We have had some oncologists asking for HER2 by IHC then reflexing for FISH if the IHC is negative or equivocal on upper GI tumors. Our pathologists have been lookin for a recommended guideline to reflex for negatives in these types of cases, but haven't found any -- is anyone aware of FISH

Re: [Histonet] HER2 on upper GI biopsies

We've been getting these requests lately too. I've been wondering if we need to monitor fixation time for these specimens, since Her2neu is a possibility. Mark Tarango On Mon, Nov 2, 2009 at 9:44 AM, Maray Weirauch mweira...@crittenton.comwrote: We have had some oncologists asking for HER2 by

[Histonet] Her2....HELP

Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work

[Histonet] Her2...help

Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work

[Histonet] Her2 fixation question

I am sending this email for a friend who is having problems posting to histonet right now. Please reply to her at carol.fie...@northside.commailto:carol.fie...@northside.com and not me. From Carol Fields She writes: Hi Netters, I am attempting to write a processing schedule (VIP) for

[Histonet] Her2 Weekend fixation

Hi Netters, For those of you doing Her2 and tracking the fixation time of the breast tissuehow do you manage week-ends? Do you have a cut off on Friday afternoons, or what procedure do you follow so techs do not come in on Sunday? Any help with this is appreciated. THX in advance, Carole

[Histonet] Her2/Neu Valadation

Dear Histonetters, How are you handling/answering the following question from the CAP checklist? Thanks in advance, Amy Georgetown Hospital System 843-527-7179 **NEW** 09/27/2007 ANP.22997 Phase I N/A YES NO If the laboratory

Re: [Histonet] Her2/Neu Valadation

: [Histonet] Her2/Neu Valadation To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 4, 2008, 11:09 AM Dear Histonetters, How are you handling/answering the following question from the CAP checklist? Thanks in advance, Amy Georgetown Hospital System 843-527-7179 **NEW