Re: [Histonet] Help! Curled cryosections

2010-09-15 Thread Teresa Iglesias
Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks! -Teresa On Wed, Sep 15, 2010 at

[Histonet] Help! Curled cryosections

2010-09-15 Thread Teresa Iglesias
Is there any way to relax curled brain sections? They've been in > cryoprotectant and I tried sitting them in PBS for a while. They're to be > used in an IHC- can they be rescued? > > > >> Thanks, >> -- >> Teresa Iglesias >> Graduate Group in Animal Behavior >> University of California-Davis >> >>