Re: [Histonet] Hematoxylin Precipitate

2015-09-23 Thread Manfre, Philip via Histonet
To: gayle.cal...@bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue

Re: [Histonet] Hematoxylin Precipitate

2015-09-23 Thread Bob Richmond via Histonet
The alum hematoxylins I've worked with required daily filtration. An iridescent scum on top of the hematoxylin means you're overdue to filter. When you brew a fresh batch of alum hematoxylin, it's a light yellow color. As it oxidizes (slowly if you depend on atmospheric oxygen, instantly if you

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Gayle Callis via Histonet
: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Manfre, Philip via Histonet
] Sent: Tuesday, September 22, 2015 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Tim H via Histonet
a Cheasty" <cheas...@svm.vetmed.wisc.edu> > To: "Histonet (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu>

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Elizabeth Chlipala via Histonet
5 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Tim H via Histonet
y no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.cal...@bresnan.net > To: thiggin...@msn.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 &

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Elizabeth Chlipala via Histonet
, CO 80504 -Original Message- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 3:04 PM To: gayle.cal...@bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you

Re: [Histonet] Hematoxylin Precipitate and filtering Gill formulations

2015-09-22 Thread Gayle Callis via Histonet
@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 12:26 PM To: Elizabeth Chlipala <l...@premierlab.com>; Tim H <thiggin...@msn.com> Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Hematoxylin P

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Michael Ann Jones via Histonet
Yes, me too. Turquois blue bacteria looking guys. Trying to filter and use within a couple of days. Has anyone mentioned this to their vendor? Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Alida Bailleul via Histonet
Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used for histological processing? I have never used this product, only absolute pure ethanol. Please advise. Thank you very much All the best

[Histonet] Hematoxylin Precipitate

2015-09-21 Thread Sandra Cheasty via Histonet
Hello all, Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water

Re: [Histonet] Hematoxylin Precipitate

2015-09-21 Thread nancy lowen via Histonet
Yes, I have had the same experience.Filtering  it seems to help. On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet wrote: Hello all,                 Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the