[Histonet] How to fix frozen section?

2012-11-06 Thread Amy Lee
Hello,   I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me?     Thanks,   Amy

RE: [Histonet] How to fix frozen section?

2012-11-06 Thread Sebree Linda A
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 1:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello,   I will receive some frozen tissue sections that are not fixed. I rarely do IHC

RE: [Histonet] How to fix frozen section?

2012-11-06 Thread Connolly, Brett M
- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 2:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello,   I will receive some frozen tissue sections that are not fixed. I

Re: [Histonet] How to fix frozen section?

2012-11-06 Thread Rene J Buesa
I used acetone René J. From: Amy Lee amylee...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Sent: Tuesday, November 6, 2012 2:41 PM Subject: [Histonet] How to fix frozen section? Hello,   I will receive some frozen tissue sections that are not fixed

Re: [Histonet] How to fix frozen section?

2012-11-06 Thread arjun ruhella
i use 1:1 Acetone : alcohol. On Nov 6, 2012, at 2:41 PM, Amy Lee wrote: Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could