Hi Bret,
If you grab the first section as it comes off the blade with a pair of
forceps, then your ribbon never needs to touch the blade holder at all.
Just don't use your fingers!
Good luck,
-Eric
On Thu, Apr 21, 2011 at 12:07 PM, Clough, Bret
wrote:
> While sectioning paraffin embedded tissue
Another culprit is static. Moisture helps to keep the static down.
"Clough, Bret"
Sent by: histonet-boun...@lists.utsouthwestern.edu
04/21/2011 12:11 PM
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Subject
[Histonet] How to keep paraffin sections from sticking to blade/blade
holder.
While
Open a beer to the science gods.
It may also help to follow the other instructions sent to the histonet list.
Emily
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
On Thu, Apr 21, 2011 at 3:07 PM, Clo
Keep everything cold, cold, cold. The block, the blade, the blade holder.
Cold spray the blade and blade holder, cool the block on ice. Warning: Use
cold spray sparingly on the block, you can ruin your tissue with too much.
Hullabaloo, Caneck! Caneck
1- change cutting angle
2- keep the blade holder clean (without paraffin)
3- make sure the blade is not blunt
René J.
--- On Thu, 4/21/11, Clough, Bret wrote:
From: Clough, Bret
Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade
holder.
To: "Histonet list
While sectioning paraffin embedded tissue on a rotary microtome I noticed the
sections sticking to the blade holder and bunching up. What do I need to do so
that my section come off the blade in a ribbon? Please note that this is my
first time in sectioning tissue and this is a learning exercis
