Thanks to everyone who responded. I removed the coverslip, and ran it back to
water and re applied the secondary, dab and counterstain. It worked like a
charm.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltim
Yes
René J.
--- On Wed, 10/13/10, Kimberly Tuttle wrote:
From: Kimberly Tuttle
Subject: [Histonet] IHC OOps wrong secondary
To: "histonet@lists.utsouthwestern.edu"
Date: Wednesday, October 13, 2010, 12:07 PM
Is it possible for me to remove the coverslip, run back to water and
Is it possible for me to remove the coverslip, run back to water and re-use the
slides starting with the correct secondary?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328
