: Monday, November 01, 2010 2:34 PM
To: jh...@vet.k-state.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Staining Problem
Hi,
I think you mentioned using citrate retrieval. Have you tried high
pH
retrieval with EDTA or an equivalent? I think CD79 works better with
high pH
Hi,
I think you mentioned using citrate retrieval. Have you tried high pH
retrieval with EDTA or an equivalent? I think CD79 works better with high pH
retrieval.
Amos
Message: 1
Date: Sun, 31 Oct 2010 19:29:59 +
From: Jennifer Hill
Subject: RE: [Histonet] IHC Staining Problem
To
>
> Message: 10
> Date: Fri, 29 Oct 2010 22:44:58 -0700
> From: Jennifer MacDonald
> Subject: Re: [Histonet] IHC Staining Problem
> To: Jennifer Hill
> Cc: histonet ,
>histonet-boun...@lists.utsouthwestern.edu
> Message-ID:
> >
> Content-Type: text/pla
Thanks to everyone who has responded so far. To answer the questions asked,
our controls are either being cut the same day or the day or two before, so
they are not setting for extended periods. We have also used at least three or
four different controls, all with the same results. (The contro
How far in advance are your controls being cut? Are your patient tissues
staining?
Jennifer Hill
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/29/2010 01:11 PM
To
histonet
cc
Subject
[Histonet] IHC Staining Problem
I hope someone can help me with a problem I've
I hope someone can help me with a problem I've been having with my CD79a hand
stain. This is a stain that was validated and working perfectly, when a few
months ago I started having reduced, to no staining on my controls, with the
occasional run staining a little stronger. I followed the discu
