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Paula,
What does the artefact look like?
Another cause could be partial lifting of parts of the section, allowing
reagents to become trapped under the section.
You commonly see it in thyroids where the colloid traps antibody and/or
detection reagents under the colloid giving a particulate brown
ssage-
From: Regan Fulton via Histonet
Sent: Thursday, September 29, 2022 3:42 PM
To: RENE MORALES
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC background
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Hi,
The artifact you are seeing is a well-known artifact of the Leica covertile
desig
Hi,
The artifact you are seeing is a well-known artifact of the Leica covertile
design.
It does limit the available footprint for placing samples.
Hope this helps.
Regan
Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360
Paula,
Regardless of the position of the tissue: control/ patient ‘s tissue, most
likely is the distribution of the reagents during staining, isn’t adequate.
Syringe, staining reagents expiration, calibration, etc., Have you contacted
service from Leica? Hope the solution is found,
Thanks,
Hello everyone,
We have the Bond III and we ran an S100. We placed 2 serial sections
vertically on the slide with the control tissue at the top for staining. The
patient's section at the top underneath the control tissue had background
staining, while the section on the bottom of that had no b
Hello everyone!
I was wondering if anyone has ever pre-incubated the antibody with normal
Mouse IgG to get rid of the unspecific staining. The antibody I use
crosreacts with mouse , and both human and mouse tissues stain within a
xenograft. I've heard that one can get rid of the mouse staining by
i
