[Histonet] IHC on non-charged slides

2013-05-16 Thread Dessoye, Michael J
I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The

Re: [Histonet] IHC on non-charged slides

2013-05-16 Thread Rene J Buesa
mjdess...@commonwealthhealth.net To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 16, 2013 4:49 PM Subject: [Histonet] IHC on non-charged slides I'm hoping Histonet can come through for me!  I have an unusual case that I need to run IHC on.  Antibody is Pan Keratin Cocktail from Cell

Re: [Histonet] IHC on non-charged slides

2013-05-16 Thread Debra Siena
Michael There is a procedure where you can transfer the sections to a charges slide with a product called mount quick. You can check with newcomer supply they sell it. I know of several labs that have used it on these occasions and it works well. Sent from my iPhone On May 16, 2013, at 4:50

Re: [Histonet] IHC on non-charged slides

2013-05-16 Thread Mark Tarango
Yes, if the stain can wait get some mount quick from Newcomer. There is a good chance the tissue will fall off if you proceed with the tissue on regular slides. I would wait until I get the mount quick. Everyone else reading this should order a tube now for emergencies. You will find yourself