Not really. Time in the oven and temp is usually not an issue as long as the
tissue is well fixed/processed/infiltrated.
René J.
--- On Mon, 3/12/12, Courtney Pierce wrote:
From: Courtney Pierce
Subject: [Histonet] IHC Slides
To: "histonet@lists.utsouthwestern.edu"
Date: Monday
Having problems with IHC slides not looking the same between runs. Does it have
to do with how long and at what temp they are in the oven for?
Courtney Pierce
IHC Specialist
Quintiles
Translational R&D - Oncology
Innovation
Navigating the new health
610 Oakmont Lane
Westmont, IL 60559
Office: +
In buffer at 4ºC overnight
René J.
--- On Wed, 3/2/11, sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com
Subject: [Histonet] IHC slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 2, 2011, 2:24 PM
So...I have run down slides and done antigen retrieval on my FFPE
slides
Just hold them in buffer overnight.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and
incubate at 4 degrees overnight.
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onet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC slides
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are cur
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently in antigen retrieval that has come to room
temperature. I am not going to be able to finish the IHC stains until
tomorrow. Will it be better to keep the slides in the antigen retrieval
solution at 4 degr
Hi Cynthia,
I agree with Greg, you have to re-do your staining from beginning (from AR
step) after removing the hematoxilin.
Naira
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I should add that the DAB step may also have "quenched" all of the linked
peroxidases even before the hematoxilin was added (which would finish off any
that remained).
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box
Cynthia,
Hematoxylin will inactivate the peroxidase (link) in the tissues. You must go
back and re-do the link step and hopefully the antigenic sites will still
accept more streptavidin (or similar) conjugated antibody.
Cheer.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept.
estern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Monday, January 24, 2011 7:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ihc slides
Happy Monday Histonetters
I need to pick the brains of the IHC experts out there in histoland. I run
IHC o
-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Monday, January 24, 2011 5:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ihc slides
Happy Monday Histonetters
I need to pick the brains of the IHC experts out there in histoland. I run
IHC on the Dako autostainer. On one of our
Happy Monday Histonetters
I need to pick the brains of the IHC experts out there in histoland. I run
IHC on the Dako autostainer. On one of our runs there was not enough DAB to
dispense on all the slides. My tech dehydrated the slides and cover slipped
them. We printed the stain log on the run and
Tony, thanks for your very helpful common-sense approach to answering my
questions. It seems that baking time needs to be as standardized,
well-controlled and well-documented as other steps in the IHC process.
Especially given the importance of standardizing results in predictive and
prognost
ocked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon,
Eric
Sent: Thursday, 22 April 2010 5:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
Tony's answer (62 degrees C for 25 mins) is the minimum to ensure section
adheres to slide. We all have to stain slides that have been baked longer than
30 mins. However, especially for Her2, longer baking times can result in false
negatives or at least diminished staining. The justification
Laurie Colbert
Sent: Wednesday, 21 April 2010 12:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven
I'm curious as to whether or not others put their IHC slides on a hotplate
after cutting? If so, at what temperature and for how long? Who only uses an
:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven
I'm curious as to whether or not others put their IHC slides on a
hotplate after cutting? If so, at what temperature and for how long?
Who only uses an oven - and what temp and for how long? Does anyone u
We use an oven only at 60c for 20 minutes.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, April 20, 2010 9:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
I'm curious as to whether or not others put their IHC slides on a
hotplate after cutting? If so, at what temperature and for how long?
Who only uses an oven - and what temp and for how long? Does anyone use
both the hotplate and the oven?
Laurie Colbert
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