[Histonet] Immunofluorescence on paraffin sections

We do quite a number of renal biopsies and sometimes the frozen tissue is not sufficient for the immunofluorescent panel and we have to use paraffin sections for our fluorescence staining. It has never really worked all that well and so we are looking for alternative methods. We get all

re: [Histonet] Immunofluorescence on FFPE skin

To me, the critical data is: which Abs do you want to use on FFPWS of skin, please? If you do chromogen IHC on your skins already, with them Abs, you can do IF. Sure, autofluorescence can be a problem but..not that big a problem. Interestedly, Carl NB: that article is..strange. Carl

AW: [Histonet] Immunofluorescence on FFPE skin

. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Martin, Erin Gesendet: Montag, 18. März 2013 19:28 An: histonet Betreff: [Histonet] Immunofluorescence on FFPE skin Hello all, My

[Histonet] Immunofluorescence on FFPE skin

Hello all, My pathologist gave me a copy of Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like

[Histonet] immunofluorescence on methyl methacrylate embedded human bone samples

Dear Histonetters, Would there be a problem with autofluorescence in bone when performing immunofluorescence on formalin-fixed methyl methacrylate-embedded human bone marrow trephines? Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for

[Histonet] Immunofluorescence in the clinical laboratory, some questions

Dear Histonetters, I know that immunofluorescence has been done for decades on renal biopsies, but am curious if laboratories are using IF more these days? If so, I would be very interested to talk to you one on one about this as I have more questions on why you deviate from standard

Re: [Histonet] Immunofluorescence in the clinical laboratory, some questions

Hi Gayle, We perform IF on renal biopsies and dermatological specimens with various bullous disorders. We perform IgA, IgM, IgG, C3, C1q, Albumin, Fibrinogen, Kappa and Lambda on acetone fixed frozen sections. These are all direct IF. We perform C4D indirectly on frozen tissue due to the lack

AW: [Histonet] Immunofluorescence in the clinical laboratory, some questions

@lists.utsouthwestern.edu Betreff: [Histonet] Immunofluorescence in the clinical laboratory, some questions Dear Histonetters, I know that immunofluorescence has been done for decades on renal biopsies, but am curious if laboratories are using IF more these days? If so, I would be very interested

[Histonet] Immunofluorescence

Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] immunofluorescence mounting medium?

to compensate for the refractive index. Regards, Geert the Netherlands Message: 6 Date: Mon, 5 Mar 2012 09:33:38 -0800 From: Collette, Nicole M. collet...@llnl.gov Subject: [Histonet] immunofluorescence mounting medium? To: histonet@lists.utsouthwestern.edu histonet

[Histonet] immunofluorescence mounting medium?

Hello, Esteemed Histonetters, I am trying to get nice publication-quality images of my immunofluorescent tissue sections. I am currently using Prolong Gold, and after I let the stuff cure for several days, my 100X oil-immersion images are still smeary, even after taking into account the

RE: [Histonet] immunofluorescence mounting medium?

@lists.utsouthwestern.edu CC: Subject: [Histonet] immunofluorescence mounting medium? Hello, Esteemed Histonetters, I am trying to get nice publication-quality images of my immunofluorescent tissue sections. I am currently using Prolong Gold, and after I let the stuff cure for several days, my

[Histonet] Immunofluorescence

Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st antibody, only 2nd one, simple staining,

RE: [Histonet] Immunofluorescence

, 2012 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary

RE: [Histonet] Immunofluorescence

@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st

Re: [Histonet] Immunofluorescence staining/minimizing background staining

:50 PM Subject: [Histonet] Immunofluorescence staining/minimizing background staining Hi, We are performing some immunofluorescence staining on mouse lung tissue.  We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize

[Histonet] Immunofluorescence staining/minimizing background staining

Hi, We are performing some immunofluorescence staining on mouse lung tissue. We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize the amount of background staining we are getting. We are titering our primary

[Histonet] Immunofluorescence and FISH Combined stating

Where can I find a procedure for dual staining of blood and bone marrow smears using Vector anti-kappa and anti-lambda tagged with Coumarine followed by FISH hybridization. I am able to see what I think are plasma cells by their fluorescent blue cytoplasm and on the same slide (changing filters) I

[Histonet] Immunofluorescence double labeling with two rabbit antibodies

Hello all, I using formalin fixed paraffin embedded specimens from fetuses from sheep to examine which cells are virally infected within the CNS. I am using IBA-1 to identify microglial cells. The IBA-1 antibody is a rabbit antibody and it works really well in my specimens. At the same time I

RE: [Histonet] Immunofluorescence double labeling with two rabbitantibodies

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aline Rodrigues Sent: Tuesday, October 12, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence double labeling with two rabbitantibodies Hello all, I using formalin fixed paraffin embedded specimens from

[Histonet] Immunofluorescence QC

We have always done IF's utilizing internal positive controls. The new CAP checklist is now requiring positive and negative controls. What are you guys doing for negative controls? CONFIDENTIALITY NOTICE: The information in this

[Histonet] immunofluorescence labeling on JB-4 embedded tissues

I need to run some immunofluorescent labeling on some tissue which was embedded in JB-4. Does anyone have a protocol? My boss thinks I need to etch the plastic beforehand. Thanks. Joe Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis The Research Institute at