Folio biosciences is testing a process that will rejuvenate older sections to
restore degraded antigenicity for ISH. It's designed to help scientists with
exactly the problem you are describing
Email me if you want to help us test this process using your slides. No charge
of course.
Rob Day.
Dear all,
I am trying to detect cytokines in human pancreatic sections from
diabetics. The blocks are at least 1y old, the sections are usually fresh.
I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin,
staining overnight in BSS-saponin with the primary, biotinylated antibody
