[Histonet] Loss of Matrigel material during staining

2012-04-05 Thread Amy Porter
To all: I have a client that we are attempting to stain H E / as well as immuno on FFPE mouse skin with matrigel plugs. These things section like a dream and then when we attempt to stain only the matrigel part of the sample is falling off. Does anyone have any tricks with this stuff they

Re: [Histonet] Loss of Matrigel material during staining

2012-04-05 Thread Carrie Schray
My experience with this is that the processing schedule is too long and probably too much heat. I had some skin that was grown in a well plate from human cells processed on a biopsy run, they cut fantastic then the gel rolled onto itself during staining. Once we reduced the processing schedule and