To all: I have a client that we are attempting to stain H E / as well as
immuno on FFPE mouse skin with matrigel plugs. These things section like a
dream and then when we attempt to stain only the matrigel part of the sample
is falling off. Does anyone have any tricks with this stuff they
My experience with this is that the processing schedule is too long
and probably too much heat. I had some skin that was grown in a well
plate from human cells processed on a biopsy run, they cut fantastic
then the gel rolled onto itself during staining. Once we reduced the
processing schedule and