Hello,
Does anyone have a luciferase or RFP protocol for labeling cells in vitro?
Thank you
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC
You have to soak it in holy
Perfect!! And I was afraid Halloween was going to sneak by unnoticed!
Which vendor sells holy water, 'cause I'm gonna need some...
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Donadio; Randolph-Habecker, Julie;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC
Perfect!! And I was afraid Halloween was going to sneak by unnoticed!
Which vendor sells holy water, 'cause I'm gonna need some...
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OOOH!!! Why didn't I think of that! Good goin'!
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, Bill
Sent: Wednesday, November 02, 2011 8:19 AM
To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC
Actually, I have found that holy water reduces its sensitivity, greatly
diminishes its signal and will destroy its reactivity. (Listen
At times like these, I wonder where Joe Nocito is...
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-Habecker, Julie;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC
At times like these, I wonder where Joe Nocito is...
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! This project has been hell!
Thanks,
Julie
Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: Beckham, Sharon s...@stowers.org
Subject: RE: [Histonet] Luciferase IHC
To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio
one_angel_sec...@yahoo.com, Randolph-Habecker, Julie
You can make your own holy water. You put regular water on a stove and boil the
hell out of it!
Mike Titford
USA - Pathology
Mobile AL
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And it's not even Friday!
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio
Sent: Tue 11/1/2011 7:00 PM
To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC
You have to soak
the storm.
Cultivate it in PRAYER!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
mtitf...@aol.com
Sent: Wednesday, November 02, 2011 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet
Has anyone had good results staining for luciferase in FFPE tissue? If
so, what antibody did you use.
Thanks!!
Julie
Julie Randolph-Habecker, Ph.D.
Director, Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N, DE-360
Seattle WA
You have to soak it in holy water, then all H311 will break out
Sorry, I couldnt resist.
From: Randolph-Habecker, Julie jhabe...@fhcrc.org
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC
...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I don't know any one
exists, but I can be wrong.
René J.
--- On Thu, 6/30/11, sgoe...@mirnarx.comsgoe...@mirnarx.com
wrote:
From: sgoe...@mirnarx.comsgoe...@mirnarx.com
Subject: [Histonet] Luciferase
To: histonet
Can you stain for luciferase in tissue?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
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You would have to have an antibody to it and I don't know any one exists, but I
can be wrong.
René J.
--- On Thu, 6/30/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] Luciferase
To: histonet@lists.utsouthwestern.edu
Date
: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] Luciferase
To: histonet@lists.utsouthwestern.edu
Date: Thursday, June 30, 2011, 11:55 AM
Can you stain for luciferase in tissue?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite
, June 30, 2011 12:13 PM
To: histonet@lists.utsouthwestern.edu; sgoe...@mirnarx.com; Rene J Buesa
Subject: Re: [Histonet] Luciferase
ok there is a really BAD religious joke coming soon, isn't there?
Rene J Buesa rjbu...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I
30, 2011 11:13 AM
To: histonet@lists.utsouthwestern.edu; Sarah Goebel; Rene J Buesa
Subject: Re: [Histonet] Luciferase
ok there is a really BAD religious joke coming soon, isn't there?
Rene J Buesa rjbu...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I don't
11:13 AM
To: histonet@lists.utsouthwestern.edu; sgoe...@mirnarx.com; Rene J Buesa
Subject: Re: [Histonet] Luciferase
ok there is a really BAD religious joke coming soon, isn't there?
Rene J Buesa rjbu...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I don't know any
Question: Can you stain Luciferase in tissues?
Answer: Yes, but it's devilishly hard to do.
Sorry, I couldn't help myself.
Tom
Tom Wells BSc, ART
Faculty
School of Medical Laboratory Sciences
British Columbia Institute of Technology
Burnaby, BC
Canada
Phone: 604-412-7594
Cell:
Question: Can you stain Luciferase in tissues?
Answer: Yes, but the background noise is hell!
On Thu, Jun 30, 2011 at 12:23 PM, Tom Wells tom_we...@bcit.ca wrote:
Question: Can you stain Luciferase in tissues?
Answer: Yes, but it's devilishly hard to do.
Sorry, I couldn't help myself.
Tom
J.
--- On Thu, 6/30/11, sgoe...@mirnarx.comsgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.comsgoe...@mirnarx.com
Subject: [Histonet] Luciferase
To: histonet@lists.utsouthwestern.edu
Date: Thursday, June 30, 2011, 11:55 AM
Can you stain for luciferase in tissue?
Sarah Goebel-Dysart, BA, HT
...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I don't know any one
exists, but I can be wrong.
René J.
--- On Thu, 6/30/11, sgoe...@mirnarx.comsgoe...@mirnarx.com
wrote:
From: sgoe...@mirnarx.comsgoe...@mirnarx.com
Subject: [Histonet] Luciferase
To: histonet
F- 215-993-6803
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Thursday, June 30, 2011 11:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luciferase
Can you
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