Re: [Histonet] MYOD1

2011-09-12 Thread Troutman, Kenneth A
Hi Donna, I had a time with this one as well. I use Dako's antibody on the Leica Bondmax. I run it at 1:50 using ER2 for 30 minutes. I would be careful going any stronger on the dilution, I got quite a bit of background with a 1:40, the 1:50 stains clean but can be a little weak if you

[Histonet] MYOD1

2011-09-09 Thread Donna Liaros
Any protocol recommendations for MYOD1? I am having a difficult time in trying to work up this antibody. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories