Dear all,
I did a Masson's Trichrome Stain in frozen liver samples with cancer and it
turns quite well to differentiate the collagen in the tumour from the
normal liver but I would also like to see better the nuclei detail and have
a less intense pink.
Here is the protocol that I am using with the
I am having a problem with Biebrich Scarlet. I am attempting to do a
Masson's stain on some mouse heart tissue. Instead of the strikingly
bright red of the muscle tissue I am getting a purple color instead.
Everything else looks fine. The hearts are fixed in Penfix and are
embedded in paraffin.