06, 2017 9:28 PM
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs
sodium)
You can use either the sodium or potassium salt. Both can also be used to make
Schiff's reagent as well. In fact, many histotechs lea
You can use either the sodium or potassium salt. Both can also be used
to make Schiff's reagent as well. In fact, many histotechs leave out the
sulphite rinse step and simply rinse off in water and wash well with tap
water. It seems to work just as well.
Bryan Llewellyn
Angela Lamberth via
int, PA 19486
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> 215-652-9750
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> philip_man...@merck.com
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> -Original Message-
> From: Angela Lamberth via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> Sent: Monday, March 06, 2017 12:58 PM
> To: histonet@lists.utsouth
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)
In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is
0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse
is given as sodium metabisulfite (Carson
In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is
0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse
is given as sodium metabisulfite (Carson pg 149).
My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse
as sodium metabisulfite
