[Histonet] Mouse eyes

2009-11-23 Thread Durden, Kelley
My question concerns mouse eyes. Can anyone send me their fixation suggestions? We are receiving mouse eyes that end up with a wavy look instead of a nicely defined C and have no explanation for this occurrence. Can anyone send me their mouse eye processing schedule? We have a protocol that

Re: [Histonet] Mouse eyes

2009-11-23 Thread Roger Moretz
10:44:21 AM Subject: [Histonet] Mouse eyes My question concerns mouse eyes. Can anyone send me their fixation suggestions?  We are receiving mouse eyes that end up with a wavy look instead of a nicely defined C and have no explanation for this occurrence. Can anyone send me their mouse eye

RE: [Histonet] Mouse eyes

2009-11-23 Thread gayle callis
@lists.utsouthwestern.edu Subject: Re: [Histonet] Mouse eyes Altho' retired for 2 years, we had a protocol that retained all parts of the eye quite well.  Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C for overnight.  Don't extend the fixation as this will cause the tissue to be too hard.  Wash for about

[Histonet] Mouse Eyes

2009-07-29 Thread Jo-Ann Bader, Ms.
We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada

Re: [Histonet] Mouse Eyes

2009-07-29 Thread Rene J Buesa
-Ann Bader, Ms. jo-ann.ba...@mcgill.ca wrote: From: Jo-Ann Bader, Ms. jo-ann.ba...@mcgill.ca Subject: [Histonet] Mouse Eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.  The lens

Re: [Histonet] Mouse Eyes

2009-07-29 Thread Paula Pierce
, Ms. jo-ann.ba...@mcgill.ca To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 10:38:05 AM Subject: [Histonet] Mouse Eyes We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed