Jinhui
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need help with FISH staining protocol on
Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin,
frosen nothinhg worked. I know other people that did not suceeded on other
animals, I do not know anybody that could do it exept o
Dear Jinhui! We tried for a long time to perform SRY FISH for rats,
paraffin, frosen nothinhg worked. I know other people that did not suceeded
on other animals, I do not know anybody that could do it exept of somebody
in German, that worked with special plasmid for rat. There are not repeated
work
I have been using IDLabs porcine Y chromosome green label probe on frozen
pig tissue with good results most of the time. You say you denature the
probe and then hold it at 37oC...I would think that would allow the probe
to reanneal to itself since you also hybridize later on at that temp...try
Hi everyone,
Has anyone done FISH staining on mice tissue frozen sections for Y chromosome?
I need help with a successful protocol.
Recently I started to do FISH staining on mice liver frozen sections for
chromosome Y, but got no positive results even on male samples yet. The probe I
used was
