tochemistry J Clin Pathol 50:422–428
>
> Gudrun Lang
> Kepleruniversitätsklinik Linz, Austria
>
> -Ursprüngliche Nachricht-
> Von: Eileen Akemi Allison via Histonet [mailto:
> histonet@lists.utsouthwestern.edu]
> Gesendet: Donnerstag, 8. August 2024 18:23
> An: His
n.edu]
Gesendet: Donnerstag, 8. August 2024 18:23
An: Histonet
Betreff: [Histonet] Oven Temps and baking times used for IHC's
Good morning Histo Peeps!
I am taking a poll on what various labs are using for temperature and times for
IHC’s slides which are baked in the oven. It has been my experi
visor
Children's Hospital Los Angeles
323-361-3357
bcoo...@chla.usc.edu
Sent from my mobile
From: Naira Margaryan via Histonet
Sent: Thursday, August 8, 2024 5:36:59 PM
To: Eileen Akemi Allison
Cc: Histonet
Subject: Re: [Histonet] Oven Temps and baking time
Interesting. I never baked my slides. Left air dry o/n on room. Next day
started with deparaffinization steps 2x30min. My most AR where in the
citrate buffer in the water bath with 92C(if I remember correctly) for 37
minutes then 12 minutes cooling at room temperature. Never had a problem.
Naira
O
Good morning Histo Peeps!
I am taking a poll on what various labs are using for temperature and times for
IHC’s slides which are baked in the oven. It has been my experience while
working at Biocare Medical, we would 1st wick excess water off the slides, then
tap the rack of slides on paper; t
