Re: [Histonet] PAN CKMNF116, picking up dendritic cells?

2012-09-25 Thread Richard Cartun
It should label dendritic cells since it reacts with cytokeratin 8. These cells have been shown to contain cytokeratin protein by immunoblotting. I use this reactivity as an internal positive control. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecim

[Histonet] PAN CKMNF116, picking up dendritic cells?

2012-09-24 Thread jAswigueAlatan
Dear Histonetters, Has anyone out there experienced problem with optimizing PanCK MNF116 antibody? Our CKMNF116 stains dendritic cells on lymph nodes using Ventana protocol: CC1 - 8 mins, Ab incubation time 16 mins, Post primary peroxidase inhib selected. Basically, it should stain cytokeratins, bu