...@eae.csic.es]
Sent: Friday, May 29, 2015 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS-diastase staining
Hi there,
I am trying to do a PAA-diastase in formalin-fixed embedded samples
(ovine liver). I would be most grateful if someone could give me a
hand in:
-Protocol
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining
Hi,
Sigma has discontinued selling type VIII-alfa amylase from barley
(catalogue ref A2771). Anyone knows any other commercial source of
this enzyme or if the other amylase (other types of alfa amylase or
beta
Benavides; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining
I have been using the spit method for years with great results. Depar slide to
water, add saliva directly to slide for 10 minutes, rinse and stain PAS as
usual. Also American MasterTech has diastase malt
Hi there,
I am trying to do a PAA-diastase in formalin-fixed embedded samples
(ovine liver). I would be most grateful if someone could give me a hand in:
-Protocol (enzyme concentration, time of digestion...) for diastase
digestion (I am assuming that PAS afterwards is the normal protocol
Sigma porcine diastase is very good.A cheaper option is very disgusting but had
been done for decades, just spit on the section but that will carry bacteria
and, although I have seen doing it, I have never done it.René J.
On Friday, May 29, 2015 10:01 AM, Julio Benavides
