t 2008 9:09 am
Subject: RE: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Diastase powder will dissolve in any water, but requires some calcium ions
o function. Thus it will not perform in distilled water, but will perform
ell using plain tap water. Also, the diastase powder from
[mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
Sent: Friday, October 24, 2008 8:38 AM
To: histonet@lists.utsouthwestern.edu; McKnight, Tanisha
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Tanisha:
Being an enzyme, you cannot use diastase in distilled water. You
- Original Message -
From: "Bryan Llewellyn" <[EMAIL PROTECTED]>
To: "McKnight, Tanisha" <[EMAIL PROTECTED]>
Sent: Friday, October 24, 2008 9:34 AM
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Try porcine pancreatic alpha
I had far more consistent results with amylase type VI (sigma) than diastase,
recommended by someone from histonet when I had problems with diastase
Ren Fail
-- Original message from "McKnight, Tanisha" <[EMAIL PROTECTED]>:
--
> Hi All:
>
> I am in the process of
AIL PROTECTED]> wrote:
From: McKnight, Tanisha <[EMAIL PROTECTED]>
Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working
To: histonet@lists.utsouthwestern.edu
Date: Friday, October 24, 2008, 11:26 AM
Hi All:
I am in the process of working up a PAS Staining protocol. The
Hi All:
I am in the process of working up a PAS Staining protocol. The PAS stain
is working fine, but I am unable to get my tissue to digest. I am using
.5g of Diastase in 50ml of DI water. I tried heating the solution in a
water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
a
