Hi Scott,
"slowly freezing on dry ice" may be your problem. In my honest opinion, you
need to freeze faster. Try a different method, such as cooled isopentane,
either with LN2 (coolest method) or dry ice. You should get better results
when you freeze them faster or colder.
Good luck,
Jo Dee
~~
Hi everyone... been having some trouble lately with my
cryopreservation. Tissue looks grossly fine before embedding in OCT but
then when I cut sections on the crystat the architecture is totally
disrupted with a lot of space in between cells. It almost looks like
the cells have become so shrunken a
