[Histonet] Pms 2 antibody trouble

2012-01-12 Thread Freeman, Carol
I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but

Re: [Histonet] Pms 2 antibody trouble

2012-01-12 Thread Karen Lahti
We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, Freeman, Carol

RE: [Histonet] Pms 2 antibody trouble

2012-01-12 Thread Amber McKenzie
...@lists.utsouthwestern.edu] On Behalf Of Karen Lahti Sent: Thursday, January 12, 2012 3:05 PM To: Freeman, Carol Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pms 2 antibody trouble We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good