Re: [Histonet] Porcessing FFPE tissue without alcohol??

via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Saturday, 26 March 2016 5:30 AM To: histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM p

Re: [Histonet] Porcessing FFPE tissue without alcohol??

[mailto:carl.ho...@kcl.ac.uk] Sent: Saturday, March 26, 2016 12:56 PM To: Elizabeth Chlipala; Joanna; Rene J Buesa Cc: histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? Thanks, Liz. If you look at fat all the time, using Osmium.you then are not sure if you use K-dichromate

Re: [Histonet] Porcessing FFPE tissue without alcohol??

Cc: Hobbs, Carl; histonet Subject: RE: [Histonet] Porcessing FFPE tissue without alcohol?? We use osmium post fixation to look at fat all of the time in mouse liver, nerve and muscle samples. It works well, sample size needs to be thin, samples are friable and can crack easily. W

Re: [Histonet] Porcessing FFPE tissue without alcohol??

, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Joanna via Histonet [histonet@lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 9:20 AM To: Rene J Buesa Cc: Hobbs, Carl; histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol

Re: [Histonet] Porcessing FFPE tissue without alcohol??

Sudan Black reacts only with protein-combined fats.René On Saturday, March 26, 2016 11:20 AM, Joanna wrote: How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet > wrote: > > The only problem I

Re: [Histonet] Porcessing FFPE tissue without alcohol??

How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet > wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a > black osmium oxidate but you will not be able to use any "standard" fat >

Re: [Histonet] Porcessing FFPE tissue without alcohol??

The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.René On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote:

Re: [Histonet] Porcessing FFPE tissue without alcohol??

Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using

Re: [Histonet] Porcessing FFPE tissue without alcohol??

To embed the tissues with paraffin you HAVE TO dehydrate the tissue. This is usually done with either ethanol of 2-propanol but essentially all dehydrants will remove fat so you are right, the way to go is going frozen sections.René On Thursday, March 24, 2016 2:24 PM, "Dessasau III, Evan

[Histonet] Porcessing FFPE tissue without alcohol??

Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol? One of the labs that send their processing to us will be doing a study examining fat. I told them if they want to look at the fat they will have to cut frozen sections but I'm