Hi Julie:
You mentioned that you are buying your fixative from a commercial
supplier but that
My first thought is that there was difference in formalin... but that is not the
case.
How do you know? You did not make the fixative solution so you really have no
idea. Not to berate Fisher
] Problems with mouse brains
have very light HE staining: first you should check if either your eosin
or
haematoxilin are over used
nuclear bubbling: can be a lot of things but can be inclusion.
Brain sections are big so make at least a 24 h inclusion process.
and extensive tissue cracking: could
2009/4/3 Randolph-Habecker, Julie jhabe...@fhcrc.org
Folks,
I need some input on a problem we're seeing with some mouse brain
samples. The samples are from new born mice P0 to P14 which have been
fixed in 10% NBF (Fisher brand and well within expiration date) for 72
hours. They are then
Folks,
I need some input on a problem we're seeing with some mouse brain
samples. The samples are from new born mice P0 to P14 which have been
fixed in 10% NBF (Fisher brand and well within expiration date) for 72
hours. They are then transferred to 70% etoh and processed on an 8 hour
process.
