Hey Charles,
Why are the first 2 steps of formalin that long? If you make sure your
tissue is well fixated, then there is no need for such long fixation in the
machine. You could use step 2 for just washing the formalin out, so for
only like 5 minutes. You could use that time, to add to other chem
From: Charles Riley via Histonet<mailto:histonet@lists.utsouthwestern.edu>
Sent: 3/22/2016 8:57 AM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Processing issues
We are having issues with our tissue proces
We are having issues with our tissue processing. We use the thermo shandon
excelsior processor. All steps have agitation and under vacuum. Our routine
process is as follows
1. Formalin 1hr
2. Formalin 1hr
3. 75% alcohol 30 mins
4.85% alcohol 30 mins
5. 95% alcohol 30 mins
6.95% alcohol 30 min
