Dear Carl, thank you so much for checking!
Yes, may I please see some images? And would you mind to share your
specific protocols and the brand also of the DAPI that you bought? (I am
guessing it does not matter too much though).
Thank you very very much, I really appreciate the help.
All the
Dear Alida
my emails to your msu.montan.edu get returned to me as undeliverable
I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- HIER.
Both stain nuclei very well.
The HIER-treated section gave a brighter nuclear positivity ( so needed to
lower exposure time on taking
=
<https://biologicalstaincommission.org>
From: Alida Bailleul via Histonet
Sent: June 3, 2021 7:53 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Protocol for DAPI staining on paraffin sections
Dear Histonet list,
I have made some paraffin blocks
Dear Histonet list,
I have made some paraffin blocks of demineralized bone and cartilage. I
would like to stain paraffin slides with DAPI (or another DNA stain).
Does DAPI staining (or other fluorescent DNA stain) work well on paraffin
sections? Does anyone have a protocol they would like to