.utsouthwestern.edu] On Behalf Of Clough, Bret
> Sent: Tuesday, August 05, 2014 11:55
> To: Histonet list serv.
> Subject: [Histonet] Protocol for decalcifying and processing large bone
> section.
>
> Hi everyone,
> I was hoping someone on histonet would being willing to help me by
u] On Behalf Of Clough, Bret
Sent: Tuesday, August 05, 2014 11:55
To: Histonet list serv.
Subject: [Histonet] Protocol for decalcifying and processing large bone section.
Hi everyone,
I was hoping someone on histonet would being willing to help me by either
sharing their protocol or advising
As a general rule EDTA is used for the gentle decalcification of small or
delicate bones, such as bone marrow samples. For large and dense bones, like
the one your are trying to decalcify, you should use a strong acid. In order to
prevent having to prepare the decalcifying solution I always used
24 hours in hcl
or formic acid.
JO'C
-Original Message-
From: Clough, Bret
To: Histonet list serv.
Sent: Tue, Aug 5, 2014 10:56 am
Subject: [Histonet] Protocol for decalcifying and processing large bone section.
Hi everyone,
I was hoping someone on histonet would being willing to
Hi everyone,
I was hoping someone on histonet would being willing to help me by either
sharing their protocol or advising me on decalcifying and processing large bone
sections. The bone sections are from the femural head of sheep measuring
roughly 1cm x 1 1/2 cm cube. Currently I’ve been decal
