[Histonet] Re: Anti-human nuclear antibody

2013-04-12 Thread Langsdorf, Aliete
You may also want to try the anti-NuMA antibody from cell signaling (#3888) - (rabbit polyclonal). I have found it to be an extremely clean antibody, excellent for detecting even one or two human cells lodged in mouse tissue. Good luck! ~Ally Research Technologist Comander Lab - Ocular Genomics

[Histonet] RE: Anti-human nuclear antibody

2013-04-11 Thread Elizabeth Cameron
Forgot to mention this is on FFPE tissue From: Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was

[Histonet] RE: Anti-human nuclear antibody

2013-04-11 Thread Anatoli Gleiberman
Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03), Anatoli Gleiberman,