[Histonet] RE: Frozen sections for Immunoflouresence

2014-09-02 Thread Burnett, Brandy
Are you using pre-cut control slides? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [vickroy@mhsil.com] Sent: Tuesday, September 02, 2014 10:51 AM To:

[Histonet] RE: Frozen sections for Immunoflouresence

2014-09-02 Thread Tony Henwood (SCHN)
Hi Jim, You mention that the OCT seems a little softer. Could it be that the sections are thicker than you usually produce. Check the Frozen section HE to check this. If sections are thicker they will tend to detach from the slides. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,