Judith
Its been years ago but I wrote a ASCP tech sample on this - I can't remember
what solution it was but one of the solutions we used for the GMS stain
actually grew fungus in it and we were getting staining on top of the tissue
sections also. If you don't think it's one of your solutions
Hi Judith, If you have made completely fresh solutions, even any stock reagents
and still have the problem, then the fungus may be arriving from another
source. I once had a problem of pollen landing on my water bath and showing up
on the stains that would detect it. You may have fungus on
and delete the message completely from your
computer system.
--
Message: 9
Date: Tue, 4 Feb 2014 09:11:35 -0700
From: Elizabeth Chlipala l...@premierlab.com
Subject: [Histonet] RE: Fungus contamination
To: Pardue, Judith judith_par...@memorial.org,
histonet
] On Behalf Of Mayer,Toysha N
Sent: Tuesday, February 04, 2014 10:33 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Fungus contamination
Judith,
What counterstain are you using for each? If it is the light green, then that
may be the culprit. Also check your baskets that you
The solution that grows fungus is the light green counterstain.
Are you using that for your PAS and the GMS? When we did PAS for fungus, we
used light green instead of hematoxylin to counterstain.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
