Several questions:
1. Like Pamela has asked - what's the processing schedule and what
regents are used? I've had some problems if the brain is soaked in EtOH too
long (70% or higher), it will cause chattering.
2. Do you slice the whole brain including cerebellum and spinal cord
Please give your processing schedule with times and reagents? It is hard to
help without knowing how the tissue was handled from sacrifice to embedding.
Pam Marcum
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