One other possiblity to consider is a thinner section at the bottom. That
could be the answer if the problem is intermittent even within the same
staining rack. If the section is thinner, the staining appears paler.
Good luck discovering the cause!
_
affin off the
slides before de-par!
Josie Britton HT
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang
Sent: Wednesday, November 19, 2008 9:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: Uneven H&E Staining
02:34
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] RE: Uneven H&E Staining
Recently we have had an issue with uneven H&E staining. Lately slides that
have
three sections on one slide, mostly GI biopsies, and the last section
(bottom of
slide) are paler than the upper two
rrington
Sent: Wednesday, 19 November 2008 12:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Uneven H&E Staining
Recently we have had an issue with uneven H&E staining. Lately slides that
have
three sections on one slide, mostly GI biopsies, and the last section (b
Recently we have had an issue with uneven H&E staining. Lately slides that
have
three sections on one slide, mostly GI biopsies, and the last section (bottom
of
slide) are paler than the upper two section. All cut by the same person and
the
stains were great. What could be the cause of thi
