Laurie, it can be a designee, but that should be detailed in your validation
procedure so it is clear the designee is acting for the Technical Director.
Tim Morken
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.ed
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 11, 2013 1:09 PM
To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Validation
: Wednesday, November 16, 2011 4:42 PM
To: 'Kim Donadio'; Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Validation
Kim wrote: "Seems the actual amount of validation slides can vary with
different Medical directors( Pathologist)"
Yes, validation is s
n Francisco, CA, USA
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com]
Sent: Wednesday, November 16, 2011 12:42 PM
To: Morken, Timothy; 'Amber McKenzie'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Validation
Seems the actual amount of validation slides c
utsouthwestern.edu"
Sent: Wednesday, November 16, 2011 2:08 PM
Subject: [Histonet] RE: Validation
Amber, is this new instrument the same kind as your old instrument and are you
using the same reagents? Or is it a totally different platform with different
reagents?
Putting in a new instrument of th
Amber, is this new instrument the same kind as your old instrument and are you
using the same reagents? Or is it a totally different platform with different
reagents?
Putting in a new instrument of the same kind and using the same reagents just
requires verifying the new instrument works. You h
Amber
When you optimize a stain you are normally just looking at one tissue type or
possible a couple, not sure how you handle protocol development. The amount of
work that is required for validation in the clinical setting is dependent upon
the type of primary antibody that you are using. If
Optimizing is getting the stain to look a certain way based on retrieval,
incubations times etc validation is the process of running cases that are
known positives of varying levels(degrees of positivity) and running them with
normal cases to ensure the antibody is staining as intended based
