With the 100% ETOH, Histoclear, and paraffin, you will need two changes as has
already been mentioned. Dense, thick or fatty tissues require more time. Given
these are small animal tissues you may be able to cut your time in each station
in half. You also may think about using isopropanol
First of all THANK YOU for getting our field into a high school and teaching
the kids. This is a very good thing for all of us in the field.
Could you give us an idea of the size of the specimen you are attempting to
process? A great deal depends on the tissue size as to how long it can be
Hi Adelle, Biopsy sizes will matter, but you might shorten some of these times,
but be sure to add at least one more Histoclear to make sure there is no
alcohol in it and at least one more paraffin to make sure there is no
histoclear carryover.If you go back to 70% at any time you will have to
Thank you Tom and Pat-
Here are the biopsy sizes:
I have my classes experimenting with specimen size to try to find what will
work best for our situation. My 1st block class has 2mm biopsy cores, 2nd
block has 4mm biopsy cores and 3rd block has 6mm biopsy cores- each about 2- 3
mm thick.
I
