We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1
hour followed by 10 minutes in 50% Ethanol rinsing in several changes of
buffer afterward and it seems to be working well for a multitude of
autofluorescent problems on FFPE sections for us. We tried to Sodium
The copper binds with the hemoglobin in the RBC and greatly reduces or
eliminates autofluoresence depending on timing.
10 mM Copper Sulfate
10 mM Copper Sulfate
Cupric Sulfate...1.25 gm
50 mM Amonium acetate (pH5)500.0 ml
Adjust ph to 5.0 with 1.0 M NaOH
50 mM Ammonium
