I have had folding and bubbles occur periodically with PFA fixed, sucrose
cryoprotected frozen brain tissue I receive from other labs. I streak a
distilled water moistened brush across the slide then pick up the frozen
section on the moistened area. It comes out flat and smooth every time. I
Hello Terry,
These are very difficult. The colder the better. (-27). keep the slides in the
cryostat. Cut the section. Place section on a cold slide, and keep the slide in
the croystat during the following procedure.
Hold slide in left hand and brush in right. Place finger under one edge of
Terry,
Here what works for us -
Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat.
Very gently press the cold slide onto the cut section so it makes contact and
the section sticks to the slide.
Flip slide over and use your finger to warm the back of the slide
Forgot to mention that we keep the cryostat around -16 to -18 C
Brett
-Original Message-
From: Connolly, Brett M
Sent: Thursday, November 17, 2011 2:58 PM
To: 'McLaughlin, Terry '; histonet@lists.utsouthwestern.edu
Subject: RE: to cut or not to cut mouse brains
Terry,
Here what
