Hi Richard,
I would strongly recommend the Churukian and Schenk method (J.Histotechnol
4(2):85-86, 1981).
A simple but brilliant stain.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the
Richard
We use 0.04% T. Blue in an acetate buffer that is pH'd to 4.0. Stain for about
5 minutes, rinse in water, let air dry and then coverslip in xylene. You will
loose the metachromatic staining if you run the slides through alcohol. I have
an SOP if you would like to see one.
Liz
