To All:
We have been experiencing problems sectioning mouse brain. The sectioning is
fine, but we have problems in the water bath. At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections
We keep our water bath at 40-41 C. Sometimes we add some 70% ethanol to the
water bath and this helps to get the wrinkles out (especially for cerebellum
and mice brain). Most of our tissue is bovine brain but we deal with multiple
other species including mice. We use McCormick Paraplast Plus
